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human p300 gene  (Addgene inc)


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    Structured Review

    Addgene inc human p300 gene
    Acyltransferase <t>p300</t> catalyzes GAPDH K251-Su. A, Expression of GAPDH K251-Su was detected after siRNA on the succinyltransferases GCN5, CPT1A, DLST, and p300/CBP. Designed three siRNAs for each gene were siRNA 1#, 2#, and 3#. The controls without added siRNA are labeled as “–.
    Human P300 Gene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human p300 gene/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    human p300 gene - by Bioz Stars, 2026-05
    93/100 stars

    Images

    1) Product Images from "Tobacco Smoking Rewires Cell Metabolism by Inducing GAPDH Succinylation to Promote Lung Cancer Progression"

    Article Title: Tobacco Smoking Rewires Cell Metabolism by Inducing GAPDH Succinylation to Promote Lung Cancer Progression

    Journal: Cancer Research

    doi: 10.1158/0008-5472.CAN-24-3525

    Acyltransferase p300 catalyzes GAPDH K251-Su. A, Expression of GAPDH K251-Su was detected after siRNA on the succinyltransferases GCN5, CPT1A, DLST, and p300/CBP. Designed three siRNAs for each gene were siRNA 1#, 2#, and 3#. The controls without added siRNA are labeled as “–.
    Figure Legend Snippet: Acyltransferase p300 catalyzes GAPDH K251-Su. A, Expression of GAPDH K251-Su was detected after siRNA on the succinyltransferases GCN5, CPT1A, DLST, and p300/CBP. Designed three siRNAs for each gene were siRNA 1#, 2#, and 3#. The controls without added siRNA are labeled as “–." B, Confocal assay to detect the colocalization of GAPDH (green) and p300 (red) in A549 and NCI-H2170 cells. DAPI (blue), nucleus. Scale bar, 20 μm. C, Co-IP and immunoblotting assay to test the interaction of succinyltransferases GCN5, CPT1A, DLST, and p300/CBP with GAPDH in A549 cells. D, Co-IP and immunoblotting were used to analyze the interaction of expressed His-p300 and Flag-GAPDH. E and F, The levels of GAPDH K251-Su ( E ) and GAPDH enzyme activity ( F ) were detected after overexpression of p300 in GAPDH WT, K251R-mutant, and K251E-mutant A549 cells, respectively. The OD value indicates the amount of NADH catalyzed by GAPDH ( n = 3). G, Cells were treated with 2 μmol/L acyltransferase inhibitor A485 and then added with 0.5 and 6 mmol/L glutamine for 24 hours. The effect of A485 on the expression of GAPDH K251-Su was detected. Hypoxic nutrient deficiency: cells were cultured in 1% FBS and 0.5 g/L glucose medium and an incubator at 2% O 2 . H, Bioinformatics analysis from TCGA and cBioPortal database to compare the gene (violin diagram) and protein (box diagram) expression of p300 in the tumor tissues from smoker and nonsmoker patients with NSCLC. The comparison of p300 gene expression also includes the data of adjacent normal tissues. Data are presented as mean ± SD. *, P < 0.05; ****, P < 0.0001; ns, nonsignificant. Statistical significance was determined by an unpaired t test. See also Supplementary Fig. S8. WCL, whole-cell lysate.

    Techniques Used: Expressing, Labeling, Confocal Assay, Co-Immunoprecipitation Assay, Western Blot, Activity Assay, Over Expression, Mutagenesis, Cell Culture, Comparison, Gene Expression



    Similar Products

    human p300 gene  (Addgene inc)
    93
    Addgene inc human p300 gene
    Acyltransferase <t>p300</t> catalyzes GAPDH K251-Su. A, Expression of GAPDH K251-Su was detected after siRNA on the succinyltransferases GCN5, CPT1A, DLST, and p300/CBP. Designed three siRNAs for each gene were siRNA 1#, 2#, and 3#. The controls without added siRNA are labeled as “–.
    Human P300 Gene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human p300 gene/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    human p300 gene - by Bioz Stars, 2026-05
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Acyltransferase p300 catalyzes GAPDH K251-Su. A, Expression of GAPDH K251-Su was detected after siRNA on the succinyltransferases GCN5, CPT1A, DLST, and p300/CBP. Designed three siRNAs for each gene were siRNA 1#, 2#, and 3#. The controls without added siRNA are labeled as “–.

    Journal: Cancer Research

    Article Title: Tobacco Smoking Rewires Cell Metabolism by Inducing GAPDH Succinylation to Promote Lung Cancer Progression

    doi: 10.1158/0008-5472.CAN-24-3525

    Figure Lengend Snippet: Acyltransferase p300 catalyzes GAPDH K251-Su. A, Expression of GAPDH K251-Su was detected after siRNA on the succinyltransferases GCN5, CPT1A, DLST, and p300/CBP. Designed three siRNAs for each gene were siRNA 1#, 2#, and 3#. The controls without added siRNA are labeled as “–." B, Confocal assay to detect the colocalization of GAPDH (green) and p300 (red) in A549 and NCI-H2170 cells. DAPI (blue), nucleus. Scale bar, 20 μm. C, Co-IP and immunoblotting assay to test the interaction of succinyltransferases GCN5, CPT1A, DLST, and p300/CBP with GAPDH in A549 cells. D, Co-IP and immunoblotting were used to analyze the interaction of expressed His-p300 and Flag-GAPDH. E and F, The levels of GAPDH K251-Su ( E ) and GAPDH enzyme activity ( F ) were detected after overexpression of p300 in GAPDH WT, K251R-mutant, and K251E-mutant A549 cells, respectively. The OD value indicates the amount of NADH catalyzed by GAPDH ( n = 3). G, Cells were treated with 2 μmol/L acyltransferase inhibitor A485 and then added with 0.5 and 6 mmol/L glutamine for 24 hours. The effect of A485 on the expression of GAPDH K251-Su was detected. Hypoxic nutrient deficiency: cells were cultured in 1% FBS and 0.5 g/L glucose medium and an incubator at 2% O 2 . H, Bioinformatics analysis from TCGA and cBioPortal database to compare the gene (violin diagram) and protein (box diagram) expression of p300 in the tumor tissues from smoker and nonsmoker patients with NSCLC. The comparison of p300 gene expression also includes the data of adjacent normal tissues. Data are presented as mean ± SD. *, P < 0.05; ****, P < 0.0001; ns, nonsignificant. Statistical significance was determined by an unpaired t test. See also Supplementary Fig. S8. WCL, whole-cell lysate.

    Article Snippet: The eukaryotic expression plasmid for human p300 gene (pEnCMV-6His-EP300-SV40-Neo, RRID: Addgene_228220) was obtained from MiaoLing Biology, and the plasmids and lentivirus for human GAPDH gene interference (PMT517-shGAPDH, RRID: Addgene_228221) and human WT GAPDH gene (PSE4462-GAPDH-WT-3Flag, RRID: Addgene_228221) or mutant GAPDH gene (PSE4462-GAPDH-K251R-3Flag, RRID: Addgene_228223; PSE4462-GAPDH-K251E-3Flag, RRID: Addgene_228224; and PSE4462-GAPDH-K251R/K254R-3Flag, RRID: Addgene_228225) eukaryotic expression were obtained from Sangon Biotech.

    Techniques: Expressing, Labeling, Confocal Assay, Co-Immunoprecipitation Assay, Western Blot, Activity Assay, Over Expression, Mutagenesis, Cell Culture, Comparison, Gene Expression